Horticultural performance of in vitro propagated plants of sacha inchi

O objetivo do presente trabalho foi avaliar, em condições de campo, características horticulturais de mudas propagadas in vitro de Sacha inchi (Plukenetia volubilis L), quanto à época de florescimento e colheita, características morfológicas relacionadas à coloração e forma de folhas, caule e frutos, bem como a produtividade do material propagado in vitro. As mudas de cultura de tecidos foram propagadas por seis semanas em meio de cultivo com sais e vitaminas de MS, acrescido de 30g L-1 de sacarose e 1,0mg L-1 de BAP, a partir do ponteiro de plântula germinada in vitro. Foram selecionadas, ao acaso, quatro plantas propagadas in vitro, quatro plantas por via seminal e mais vinte e duas plantas, também por via seminal, compondo a bordadura, totalizando trinta plantas. As análises foram semanais até completar quatorze meses do plantio, que ocorreu antes da primeira poda. Os resultados obtidos indicam a não ocorrência de variabilidade genética nas plantas produzidas in vitro, bem como precocidade na produção e maior produtividade, quando comparado com o material convencional obtido via seminal44610501053FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP10/50335-0; 10/17744-4The aim of this study was to evaluate, in the field, the horticultural characteristics of Sacha inchi (Plukenetia volubilis L.) in vitro plants considering time of flowering and harvesting, the productivity, as well as their morphological characteristics: color and shape of leaves, stems and fruits. The plantlets, originated from the apex of in vitro plants, were propagated in culture medium with MS salts and vitamins, add 30g L-1 sucrose and 1.0mg L-1 BAP for six weeks. Were selected at random, four plants propagated in vitro, four plants by seminal and twenty-two plants, also by composing the border, totaling thirty plants. Analyses were completed weekly until fourteen months after planting, which occurred before the first pruning. The results indicate the non-occurrence of genetic variability in vitro grown plants, as well as early in the production and increased productivity as compared with the conventional material obtained via semina

In vitro propagation of Astrocarium aculeatum

There is a large number of species within the genus Astrocaryum with the potential for use as food and for oil production. The most used species, tucumã do Amazonas, is consumed on a large scale in the States in the northern region of Brazil, originating from extractivism. Zygotic embryos of mature and immature tucumã do Amazonas (Astrocaryum aculeatum G. Mey.) seeds were inoculated in a semi-solid Murashige and Skoog (MS) culture medium supplemented with vitamins. The surviving embryos were transferred to the same MS medium supplemented with 0.0; 1.0; 3.0 and 5.0mg L-1 of BAP, obtaining an increasing rate of shoot formation.O gênero Astrocaryum possui um grande número de espécies com potencial alimentício e produtoras de óleos. A espécie mais utilizada, o tucumã do Amazonas, é consumida em larga escala nos estados da região norte do Brasil e originado do extrativismo vegetal. Embriões zigóticos de sementes maduras e imaturas de tucumã do Amazonas (Astrocaryum aculeatum G. Mey) foram inoculados em meio de cultivo semi-sólido de Murashige e Skoog (MS) suplementado com vitaminas. Os embriões sobreviventes foram transferidos para o mesmo meio MS suplementado com 0,0; 1,0; 3,0 e 5,0mg L-1 de BAP, obtendo-se taxa crescente de brotação, sendo as mais eficientes as doses de 3,0 a 5,0mg L-1555

Quality of life in temporomandibular disorder patients with localized and widespread pain

AIM: To compare temporomandibular (TMD) subgroups classified according to the presence of localized pain (LP) or widespread pain (WP) in order to assess the quality of life domains and verify which components affect most the functional capacity of facial pain patients.METHODS: A cross-sectional study was conducted and the Short Form-36 Health Survey was applied in order to assess quality of life. Thirty-nine TMD/WP patients, 37 TMD/LP patients and 40 subjects free of TMD complaints were evaluated. RESULTS: TMD/WP patients differed significantly from healthy controls in all SF-36 components and TMD/LP patients ranked between them. It was also observed that patients with bodily pain and TMD with WP are respectively, 4.16 and 49.42 times more likely to have low functional capacity. CONCLUSIONS: Functional capacity in TMD subgroups was only affected by the presence of bodily pain and WP. These patients feature high chance of low functional capacity. Furthermore, TMD patients with localized and widespread pain share role-emotional impairments133193197COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES2009-201

An in vitro assessment of type, position and incidence of isthmus in human permanent molars

Root canal anatomical complexities, such as isthmus, may limit the action of the endodontic instruments, irrigant solutions and intracanal medications, leading to endodontic treatment failure. Objectives: This in vitro study assessed the type, position and incidence of isthmus in human permanent molars. Material and Methods: One hundred and twenty eight upper and lower first and second permanent molars were analyzed. The roots were embedded in transparent resin, and then split at different distances from the apex (1.0-2.5-4.0-5.5-7.0 mm). Following the sample examination in stereomicroscope, the data were submitted to chi-square statistical test at a 5% significance level. Results: The highest isthmus incidence was at 7.0 mm from the root apex in all samples, except the distal root of lower molars (at 5.5 mm). In upper and lower molars, type V (complete isthmus with a continuous opening between the two main root canals) was the most common classification of isthmus (28.8%). In the mesial root of first and second mandibular molars, type IV had the highest incidence (36% and 23.9%, respectively). Conclusion: It was concluded that isthmus was widely found in flat roots, with a low percentage in areas close to the apex. In upper and lower molars, these structures were most frequently found at 7 mm from the apex224274281CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES302575/2009- 0sem informaçã

An in vitro assessment of type, position and incidence of isthmus in human permanent molars

Root canal anatomical complexities, such as isthmus, may limit the action of the endodontic instruments, irrigant solutions and intracanal medications, leading to endodontic treatment failure. Objectives: This in vitro study assessed the type, position and incidence of isthmus in human permanent molars. Material and Methods: One hundred and twenty eight upper and lower first and second permanent molars were analyzed. The roots were embedded in transparent resin, and then split at different distances from the apex (1.0-2.5-4.0-5.5-7.0 mm). Following the sample examination in stereomicroscope, the data were submitted to chi-square statistical test at a 5% significance level. Results: The highest isthmus incidence was at 7.0 mm from the root apex in all samples, except the distal root of lower molars (at 5.5 mm). In upper and lower molars, type V (complete isthmus with a continuous opening between the two main root canals) was the most common classification of isthmus (28.8%). In the mesial root of first and second mandibular molars, type IV had the highest incidence (36% and 23.9%, respectively). Conclusion: It was concluded that isthmus was widely found in flat roots, with a low percentage in areas close to the apex. In upper and lower molars, these structures were most frequently found at 7 mm from the apex

The effect of light-curing source and mode on microtensile bond strength to bovine dentin

PURPOSE: The purpose of this study was to evaluate the effects of different light-curing techniques on the microtensile bond strength of hybrid and packable resin composite to dentin. The null hypotheses were that different light-curing techniques do not affect the adhesion of resin composites to tooth structure and that different resin composites do not have a similar bond to dentin. MATERIALS AND METHODS: One hundred four box-shaped buccal preparations were made and dentin/enamel adhesive was applied according to the manufacturer's instructions (Single Bond 3M ESPE). A hybrid resin composite (Filtek Z250, A2, 3M ESPE) or a packable resin composite (Solitaire 2, A2, Heraeus Kulzer) were inserted in bulk and polymerized using one of these techniques (n = 13): (a) Soft-start (SS) using a halogen lamp (QTH); (b) LED low intensity; (c) Plasma arc (PAC) curing for 6 s for packable resin composite and 3 s for the hybrid resin composite; (d) Conventional (C) QTH curing for 40 s. Afterwards, specimens were thermocycled 1,000 times between 5 degrees C and 55 degrees C in tap water, and were sectioned into beams with a rectangular cross-sectional area of approximately 1 mm2. Microtensile bond strength testing was performed using a universal testing machine at a crosshead speed of 0.5 mm/min. RESULTS: Bond strength means +/- (SD) in MPa were: Filtek Z250: SSQTH = 17.9 (5.4); LED = 17.9 (6.4); PAC = 16.8 (6.8); CQTH = 16.1 (4.6). Solitaire 2: SSQTH = 12.4 (6.4); LED = 15.5 (4.3); PAC = 16.2 (4.4); CQTH = 13.8 (5.7). The data were structured in a split-plot design and analyzed by a two-way ANOVA and Tukey's tests (alpha = 0.05). CONCLUSION: The light-curing method did not significantly affect bond strengths. However, the bond strengths of the packable resin composite were significantly lower than those of the hybrid resin composite for all polymerization techniques, suggesting that the restorative material itself might be a more critical factor in adhesion than the curing method

Prolonged breastfeeding: mother s arguments

This paper aims to identify and analyze the reasons pointed by mothers to prolong breastfeeding beyond the first year of the child s life. The study involved 40 mothers whose children were treated in the Preventive Program of Research and Dental Treatment Center for Special Patients - Dental School of Piracicaba - UNICAMP. The group consisted of mothers who prolonged the breastfeeding beyond the baby s first year of life. All mothers were surveyed by a researcher using a specific questionnaire. In order to avoid information loss, the interviews were taped, then transcripted. Results showed that the main cause of the extended breastfeeding was maternal pleasure. It was also observed that the mother and infant attachment favors prolonged breastfeeding occurrence. Further studies should be carried out for more accurate functional analyses of variable that lead to extend breastfeeding or to wean.O objetivo deste estudo foi identificar e analisar as justificativas referidas por mães para prolongar o aleitamento materno de seus filhos além do primeiro ano de vida da criança. A metodologia envolveu o estudo de 40 mães cujos filhos eram atendidos pelo Centro de Pesquisa e Atendimento Odontológico para Pacientes Especiais - Cepae - da Faculdade de Odontologia de Piracicaba - UNICAMP. Para que a mãe fosse incluída no estudo, deveria estar amamentando depois do 12º mês de vida da criança. As participantes foram entrevistadas individualmente, utilizando-se um questionário específico. Todas as entrevistas foram gravadas em áudio. Os resultados mostraram que o motivo mais referido pelas mães para a manutenção da amamentação foi o prazer materno. Também foi observado que a proximidade mãe-bebê favorece o prolongamento do aleitamento. Estudos ainda são requeridos para obtenção de análises funcionais mais precisas de variáveis que levam ao prolongamento ou interrupção do aleitamento materno.27127

Multiple Stafne Bone Defects: A Rare Entity

Stafne bone defects (SBDs) are generally located in the lingual cortex, close to the mandibular angle. We report the occurrence of multiple SBDs in an asymptomatic patient, a 60-year-old man, referred to a radiology clinic to undergo examination for the purpose of implant planning. The case of multiple SBD presented here, probably the first reported in the literature, reinforces the hypothesis that some cases of SBD may be the result of a focal failure during the ossification of the mandible

Desempenho de adubos verdes e da primeira soqueira de cana-de-açúcar cultivados consorciadamente.

Os objetivos deste trabalho foram avaliar o potencial de algumas espécies de adubos verdes em integrar um sistema de produção intercalar com a primeira soqueira da cana-de-açúcar e seu efeito na produtividade, bem como sua capacidade de reciclar nutrientes e fixar nitrogênio simbioticamente. O trabalho foi desenvolvido no período de setembro de 2001 a agosto de 2003, em Piracicaba, SP, em um Latossolo Vermelho eutroférrico. O delineamento experimental foi em blocos ao acaso, com cinco repetições e nove tratamentos (oito espécies de adubos verdes e uma testemunha). A parcela experimental foi composta de cinco linhas de cana-de-açúcar com 10 m de comprimento e espaçadas de 1,40 m, plantada em setembro de 2001. Os adubos verdes foram semeados no final de novembro de 2002, nas entrelinhas da primeira soca da cana no sistema de consórcio, dois meses após o primeiro corte, em duas linhas espaçadas de 0,50 m e com 10 m de comprimento. As leguminosas apresentaram um bom desenvolvimento nas entrelinhas da cana-de-açúcar e não causaram queda de produtividade na cultura, com exceção da crotaláriajúncea que prejudicou a produtividade da cana-de-açúcar. Os resultados recomendam cultivo intercalar para cana após primeiro corte. As leguminosas apresentaram elevado potencial para fixação biológica do nitrogênio